Info • Leratiomyces erythrocephalus mycota031 v2.0

Status

[December 2022] The Leratiomyces erythrocephalus mycota031 v2.0 genome was sequenced with Oxford Nanopore at University of Otago (UO) as well as with PacBio and Illumina at JGI, then together assembled with canu at UO, and annotated with the JGI Annotation Pipeline. The transcriptome was sequenced with Illumina and assembled with Trinity at JGI, and also sequenced with PacBio and clustered with IsoSeq at JGI. The mitochondrial genome was assembled separately at JGI and is available under the Download tab.

This genome is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. Some of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 126.94
# of contigs 314
# of scaffolds 314
# of scaffolds >= 2Kbp 314
Scaffold N50 45
Scaffold L50 (Mbp) 0.87
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 3.16, 3.02, 2.63


ESTs Data set # sequences total # mapped to genome % mapped to genome
Ests est.fasta 201867996 186507436 92.4%
Other JGI_Illumina_RNA_contigs 94015 76630 81.5%
Other JGI_PacBio_RNA_consensuses 355675 347110 97.6%


Gene Models FilteredModels1
length (bp) of: average median
gene 1816 1546
transcript 1438 1193
exon 238 142
intron 77 60
description:
protein length (aa) 413 328
exons per gene 6.05 5
# of gene models 19128


Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.