Seq. Project name:
Bacteroides pyogenes DSM 20611
( Project ID: 1000028 )
Product:
Microbial Minimal Draft, Isolate
Proposal Name:
Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project
(Proposal ID: 733)
Project PI:
User Program:
CSP
Program Year:
2012
Scientific Program:
Microbial
Release Date:
2013-05-31
| Organism | |
|---|---|
| Genus/species/strain/isolate: | Bacteroides / Bacteroides pyogenes / DSM 20611 / |
| GOLD ID: | Gp0013532 |
| Contacts | |
|---|---|
| JGI: | |
| Request DNA: | Nikos Kyrpides <[email protected]> |
| General Information | |
|---|---|
|
QD/SD JGI ISOLATES QC AND ASSEMBLY REPORT -
1000028
Bacteroides pyogenes DSM 20611
1) RAW DATA:
LibraryName NumReads ReadType FileName
CXGW 6578198 2x150 6175.1.39285.TGACCA.fastq
2) STD READ FILTERING STATS:
Reads were screened against synthetic oligos used in the Illumina
sequencing process. Pairs of matching reads were removed from the
dataset.
Total input reads: 6578198 (100%)
Artifact reads removed: 55830 (0.8%)
Total reads removed: 55830 (0.8%)
Total reads remaining: 6522368 (99.2%)
3) STD READ IDENTIFICATION STATS
This step identifies contaminants but does not remove them from the dataset.
Total input reads: 6578198 (100%)
Num contam reads identified: 4 (0.0%)
- Ralstonia 4 0.00%
4) ASSEMBLY STATS:
a) Velvet assembly using VelvetOptimizer:
Assembly stats of the Velvet assembly created by the velvet
optimizer. The input reads have been filtered for artifacts.
Avg GC Content: 44.74 +/- 3.82%
Largest Contig: 220.1 KB
Main genome scaffold total: 90
Main genome contig total: 94
Main genome scaffold sequence total: 3.4 MB
Main genome contig sequence total: 3.4 MB (-> 0.0% gap)
Main genome scaffold N/L50: 13/79.4 KB
Main genome contig N/L50: 15/72.4 KB
Number of scaffolds > 50 KB: 26
% main genome in scaffolds > 50 KB: 76.3%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 90 94 3,417,882 3,417,277 99.98%
1 kb 76 80 3,407,644 3,407,039 99.98%
2.5 kb 64 68 3,387,655 3,387,050 99.98%
5 kb 59 63 3,366,848 3,366,243 99.98%
10 kb 49 52 3,300,203 3,299,737 99.99%
25 kb 39 42 3,122,853 3,122,387 99.99%
50 kb 26 29 2,608,544 2,608,078 99.98%
100 kb 9 12 1,425,306 1,424,925 99.97%
b) Allpaths + Velvet simulated read pairs:
Assembly stats of the ALLPATHS assembly. The input contains simulated
1-3 kb read pairs created from the Velvet assembly and reads that
have been filtered for artifacts.
Avg GC Content: 44.60 +/- 3.80%
Largest Contig: 210.3 KB
Main genome scaffold total: 79
Main genome contig total: 79
Main genome scaffold sequence total: 3.4 MB
Main genome contig sequence total: 3.4 MB (-> 0.0% gap)
Main genome scaffold N/L50: 14/74.9 KB
Main genome contig N/L50: 14/74.9 KB
Number of scaffolds > 50 KB: 27
% main genome in scaffolds > 50 KB: 74.1%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 79 79 3,422,915 3,422,915 100.00%
1 kb 79 79 3,422,915 3,422,915 100.00%
2.5 kb 67 67 3,402,949 3,402,949 100.00%
5 kb 62 62 3,382,279 3,382,279 100.00%
10 kb 54 54 3,326,542 3,326,542 100.00%
25 kb 42 42 3,127,052 3,127,052 100.00%
50 kb 27 27 2,535,334 2,535,334 100.00%
100 kb 8 8 1,227,313 1,227,313 100.00%
5) KEY PIPELINE CMDS:
a) Artifact removal step:
duk params: -k 22 -s 1 -c 1
b) Velvet assembly step for creating simulated read pairs:
Velvet version: 1.1.04
Velvet optimizer version: 2.1.7
Velvet optimizer params: --v --s 51 --e 71 --i 4 --t 1 --f "-shortPaired -fastq $FASTQ" --o "-ins_length 250 -min_contig_lgth 500"
c) Simulated read pairing creation step:
Wgsim version: 0.3.0
Wgsim params: -e 0 -1 100 -2 100 -r 0 -R 0 -X 0
d) ALLPATHS assembly step:
ALLPATHS version: r41043
Contents of in_libs.csv:
library_name, project_name, organism_name, type, paired, frag_size, frag_stddev, insert_size, insert_stddev, read_orientation, genomic_start, genomic_end
STD_1,project,assembly,fragment,1,200,35,,,inward,0,0
SIMREADS,project,assembly,jumping,1,,,3000,300,inward,0,0
6) WORKFLOW STEPS:
1. Removed illumina artifacts (synthetic oligos used in the laboratory).
2. Created velvet assembly of the artifact filtered data.
3. Created simulated 1-3 kb read pairs using velvet contigs from step 2.
4. Created allpaths assembly using velvet simulated read pairs (step 3) and the artifact filtered data.
7) RELEASE DATE:
Tue Apr 23 10:17:45 PDT 2013 By Alexander Spunde- [email protected]
8) AUTHORS:
For additional information, please contact:
Stephan Trong - [email protected]
James Han - [email protected]
This file was automatically generated by the jigsaw pipeline software (version 2.0.7).
|
| Funding | |
|---|---|
| The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. | |
| Groups |
|---|
| ## | Name | Type |
|---|---|---|
| 1 | Bacteroidetes | |
| 2 | Host Associated |
