The ever-increasing number of sequenced genomes presents us with an exciting opportunity to discover highly conserved gene families of unknown function, then characterize them experimentally. We have detected these gene families across the kingdom Fungi (see Methods at the bottom of this page), and invite the international research community to functionally characterize their individual members and propagate their annotations across the Fungal Tree of Life. Investigators can register, login, click on any cluster below (the number of the left), and add notes to any protein from the list along with the methods used for functional characterization.

##	Genes	Expressed Genes %	Genes with Phenotypes	Unique Species	Proteins with PFAM Domains	Unique PFAM Domains Count	Protein PFAM Domains	Uniprot HMM Hint	PDB HMM Hint	AlphaFold pLDDT	Foldseek PDB Hint	Conserved In	Avg. Protein Length
1	1,641	84	1	461	0	0				78		Fungi	244
2	1,348	50	1	726	2	2	2OG-Fe dioxygenase: 1 2OG-Fe(II) oxygenase superfamily: 1	A0A093V829_TALMA	6NIE	88		Universal	356
3	1,301	85	0	356	32	2	Arrestin (or S-antigen), N-terminal domain: 31 REV protein (anti-repression trans-activator protein): 1			86		Fungi	423
4	1,127	67	1	1,067	0	0		A0A0F0IG53_ASPPA		90		Fungi and Viridiplantae	148
5	1,063	60	0	560	0	0				76		Fungi	379
6	1,045	60	0	730	0	0		A0A0F8UPA4_9EURO		82		Eukaryota	220
7	969	81	0	362	0	0		A0A074S9U8_9HOMO		75	6IBB	Fungi	326
8	967	65	0	947	0	0		A0A0M0TX97_9PEZI		81		Fungi and Viridiplantae	118
9	956	60	0	916	0	0				61		Universal	495
10	951	61	0	921	0	0			6GAP	60		Eukaryota	641
11	944	61	0	929	1	1	SOS response associated peptidase (SRAP): 1			71		Fungi and Viridiplantae	391
12	940	63	0	931	0	0			5MRC	83		Fungi and Viridiplantae	303
13	925	59	0	864	0	0		L8PHG1_STRVR		51		Universal	397
14	920	62	0	911	0	0				82		Universal	354
15	915	64	0	898	0	0				76		Fungi and Viridiplantae	183
16	913	64	0	904	2	1	Membrane-associating domain: 2	A0A0A2KXQ6_PENIT		74		Eukaryota	203
17	910	63	0	892	0	0			4WJV	58		Fungi and Viridiplantae	1,106
18	899	64	0	724	0	0				90		Fungi and Viridiplantae	114
19	897	62	0	885	0	0				50		Eukaryota	430
20	895	62	0	873	0	0				60		Fungi and Viridiplantae	501
21	895	65	0	890	0	0				67		Fungi	397
22	894	63	0	888	0	0		A0A0F8B6F2_CERFI		86		Fungi	305
23	893	62	0	885	1	1	Cytochrome c oxidase biogenesis protein Cmc1 like: 1			83		Eukaryota	323
24	888	63	0	882	0	0		G3JD84_CORMM		68		Fungi and Viridiplantae	325
25	880	61	0	840	0	0				96		Fungi	183
26	879	73	1	839	45	2	NAD dependent epimerase/dehydratase family: 25 NAD(P)H-binding: 20	A0A0F7SEX8_PHARH		91	3GPI	Fungi and Viridiplantae	299
27	872	61	0	848	0	0				45		Fungi and Viridiplantae	420
28	867	64	0	857	0	0				50		Universal	563
29	865	62	1	843	5	1	Bombesin-like peptide: 5	A0A084GFB1_9PEZI		78		Eukaryota	290
30	864	61	0	829	1	1	Atrophin-1 family: 1	A0A0L1HK43_9PLEO		58		Fungi and Viridiplantae	684
31	861	62	0	850	0	0		A0A093V143_TALMA		80		Fungi and Viridiplantae	624
32	858	60	0	834	0	0				76		Fungi and Viridiplantae	225
33	846	63	0	759	0	0		F2TB63_AJEDA		78		Fungi and Viridiplantae	227
34	842	64	0	831	0	0				61		Fungi	278
35	840	65	0	827	0	0		A0A0L1HN00_9PLEO	4BH6	58		Fungi and Viridiplantae	391
36	838	65	1	829	2	1	Peptidase family M41: 2			60		Universal	409
37	837	64	0	820	0	0				68		Fungi and Viridiplantae	590
38	837	63	0	829	0	0				57		Universal	438
39	834	62	0	793	0	0		A0A0L1HFC6_9PLEO		78		Fungi and Viridiplantae	964
40	833	59	0	815	0	0			2ALB	79		Eukaryota	263
41	831	67	0	719	0	0		G0S8C3_CHATD		91		Fungi and Viridiplantae	162
42	830	63	0	809	0	0				69		Fungi and Viridiplantae	365
43	826	64	0	805	0	0				55		Fungi and Viridiplantae	527
44	824	67	0	817	0	0		A0A0F0I9G2_ASPPA		68		Fungi and Viridiplantae	182
45	820	66	1	810	24	1	Glutathione-dependent formaldehyde-activating enzyme: 24	A0A0Q8MCB2_9CAUL	1X6M	71		Eukaryota	250
46	817	63	0	802	103	1	Altered inheritance of mitochondria protein 19: 103			79		Fungi and Viridiplantae	137
47	814	65	0	806	0	0				68		Universal	596
48	813	66	0	800	0	0				71		Fungi	284
49	812	63	0	793	0	0				69		Universal	176
50	811	64	0	782	2	1	BLOC-1-related complex sub-unit 6 C-terminal helix: 2			78		Fungi and Viridiplantae	169
51	804	64	0	767	1	1	HupE / UreJ protein: 1	A0A0D9LDS9_9EURO		87		Fungi and Viridiplantae	179
52	804	68	0	796	0	0				75		Fungi	341
53	803	65	0	798	0	0		K1WL11_MARBU		68		Eukaryota	424
54	798	59	0	779	1	1	Protein of unknown function (DUF4030): 1	C6H7A7_AJECH		68		Fungi and SAR	136
55	798	63	0	772	0	0		A0A0L1HFD3_9PLEO		71		Fungi and Viridiplantae	536
56	797	63	0	790	0	0				79		Fungi	240
57	795	62	0	786	0	0		A0A0K8LIG6_9EURO		46		Fungi and Viridiplantae	656
58	790	64	0	781	0	0		A0A0G2GCF4_9EURO		79		Eukaryota	565
59	790	65	0	784	0	0		K2RK39_MACPH		78		Fungi and Viridiplantae	295
60	789	64	0	785	0	0		A0A094D5E2_9PEZI		75		Fungi and Viridiplantae	415
61	789	58	0	780	0	0		A0A0L1HYK7_9PLEO		82		Universal	683
62	783	62	1	767	0	0		A0A0L1HQ48_9PLEO		49		Fungi and Prokaryotes	528
63	781	64	0	736	0	0		A0A0F9XFM1_TRIHA		51		Eukaryota	562
64	781	65	0	774	0	0		A0A0L1HQ04_9PLEO		82		Fungi and Viridiplantae	351
65	779	78	1	577	2	1	Fungal protein of unknown function (DUF1774): 2		2N02	90		Fungi	270
66	772	60	0	764	0	0		A0A0L1HIA9_9PLEO		81		Universal	638
67	770	65	0	764	0	0				57		Fungi and SAR	439
68	767	85	0	407	1	1	COPI associated protein: 1	A0A0G4KXT8_9PEZI		74		Fungi	279
69	762	66	0	746	0	0				53		Fungi	266
70	747	61	0	715	0	0				79		Fungi and Viridiplantae	560
71	745	66	0	743	0	0		A0A0G2ETE7_9PEZI		65		Eukaryota	451
72	739	64	0	735	0	0				71		Fungi and Viridiplantae	120
73	736	63	0	734	0	0			1WYP	69		Eukaryota	526
74	730	64	0	718	0	0		V5F996_BYSSN		73		Fungi and SAR	522
75	729	68	0	712	0	0		L7IQI8_MAGOP		64		Fungi	150
76	729	66	0	722	1	1	Prefoldin subunit: 1	S3D1R2_OPHP1	4UXV	64		Eukaryota	1,017
77	725	81	0	378	0	0		A0A074RSI5_9HOMO	4B5Q	89	5NO7	Eukaryota	319
78	723	86	0	357	0	0		A0A067NL52_PLEOS		90		Fungi	188
79	723	56	0	662	0	0				70		Fungi and Viridiplantae	342
80	720	71	0	715	0	0				56		Fungi and Viridiplantae	484
81	715	63	0	700	0	0				68		Eukaryota	289
82	697	66	0	697	0	0				63		Fungi	333
83	685	66	0	662	0	0				85		Fungi	329
84	677	66	0	670	0	0			5N3U	77		Fungi	334
85	677	62	0	672	5	3	AT hook motif: 2 Leucine Rich Repeat: 2 Leucine Rich Repeat: 1		2AST	71		Fungi and Viridiplantae	716
86	672	61	0	655	0	0				66		Fungi and SAR	284
87	668	60	0	659	8	2	F-box domain: 7 F-box-like: 1	A0A0L1IM61_ASPNO	6O60	73		Fungi	592
88	665	65	0	661	0	0			6AHU	83	6AHU	Fungi	152
89	660	59	0	651	0	0				61		Eukaryota	410
90	657	72	1	633	1	1	F-box-like: 1		5GR8	89	2Q4G	Fungi and Viridiplantae	544
91	651	57	0	619	0	0		A0A0J5PEM5_ASPFM	1QZG	71		Fungi and Viridiplantae	360
92	643	68	0	629	0	0				90		Fungi and Viridiplantae	183
93	621	60	0	612	0	0		A0A0G4KXT8_9PEZI		64		Eukaryota	271
94	614	66	0	554	0	0		J4KNZ3_BEAB2		78		Universal	823
95	612	64	0	608	0	0		A0A0G4KWN1_9PEZI		57		Fungi and Viridiplantae	1,169
96	610	65	0	601	0	0		A0A0L1HRB8_9PLEO	4WOL	57		Fungi and Viridiplantae	374
97	610	67	0	610	0	0				50		Fungi	329
98	601	66	0	574	0	0		V5HQC4_BYSSN		62		Fungi and Viridiplantae	373
99	599	57	0	590	1	1	Metchnikowin family: 1			58		Universal	240
100	594	64	0	588	0	0				78	7SDE	Fungi and Viridiplantae	569
101	594	59	0	591	0	0		Q659W9_CHICK	6TKV	81		Eukaryota	596
102	592	75	1	445	95	1	Domain of unknown function (DUF4336): 95			95		Fungi	252
103	591	67	0	586	0	0				62		Eukaryota	1,711
104	586	67	0	585	1	1	Coiled-coil domain-containing protein 34: 1			56		Fungi and SAR	408
105	580	58	1	578	0	0		A0A093VJS5_TALMA		68		Fungi and Viridiplantae	357
106	577	57	0	531	0	0				73		Eukaryota	1,096
107	576	61	0	572	0	0		W4S7N2_9XANT		72		Universal	325
108	571	64	0	562	0	0		A0A0B1P0W1_UNCNE		79		Fungi and Viridiplantae	218
109	566	51	0	294	0	0				71		Fungi	393
110	563	66	0	560	0	0		A0A0L1HSB2_9PLEO		58		Fungi and Viridiplantae	233
111	558	66	0	557	0	0		A0A093V0T1_TALMA		72		Fungi and Viridiplantae	308
112	551	84	0	345	0	0				82		Fungi	182
113	544	68	0	531	1	1	AN1-like Zinc finger: 1			63		Fungi and Viridiplantae	156
114	541	65	0	534	2	1	Small acid-soluble spore protein P family: 2	A0A0F4Z6E8_TALEM		58		Fungi and Viridiplantae	435
115	541	60	0	524	1	1	Protein of unknown function (DUF1698): 1	A0A0L1HIK1_9PLEO	3DMG	93		Fungi and Viridiplantae	397
116	539	68	0	537	0	0		A0A0M9F2P2_9HYPO	6VQB	75		Fungi and Viridiplantae	236
117	535	67	0	529	1	1	Insulinase (Peptidase family M16): 1	A0A0L1HFC6_9PLEO		81		Fungi	771
118	531	70	0	529	0	0			6QL5	79		Fungi and Viridiplantae	172
119	527	63	0	520	0	0		R1GHE5_BOTPV		70		Fungi	117
120	510	62	0	505	0	0		A0A0N1HHC8_9EURO		55		Fungi and Viridiplantae	641
121	510	87	0	354	0	0		E5R1L2_ARTGP		90		Fungi and Viridiplantae	203
122	508	62	0	501	0	0			6K15	58		Eukaryota	866
123	505	65	0	499	0	0				70		Fungi and Viridiplantae	456
124	499	62	0	481	0	0		A0A093VC35_TALMA		78		Universal	379
125	494	69	0	493	5	2	PPR repeat: 4 Pentatricopeptide repeat domain: 1		6SGA	76		Fungi and Viridiplantae	806
126	478	72	0	465	0	0				73		Fungi and Viridiplantae	167
127	465	55	0	411	0	0				53		Fungi	599
128	464	81	1	389	0	0				93		Fungi	176
129	461	69	0	457	12	4	F-box domain: 9 Protein of unknown function (DUF2442): 1 Serum albumin family: 1 F-box-like: 1			78		Fungi and Viridiplantae	507
130	460	70	1	429	0	0				85	8D9O	Fungi	192
131	454	84	0	399	303	1	Protein of unknown function (DUF1308): 303	A0A074S6E1_9HOMO		80		Fungi	610
132	450	58	0	423	0	0		A0A0F0IGJ6_ASPPA		56		Fungi and SAR	388
133	448	71	0	321	0	0				92		Fungi	160
134	444	64	0	443	0	0				78		Fungi	411
135	443	66	0	439	0	0		A0A0F0IAZ8_ASPPA	4A3T	87	4A3T	Fungi and Viridiplantae	634
136	438	86	0	347	20	1	4'-phosphopantetheinyl transferase superfamily: 20	A0A0B7FI86_THACB	2BYD	92	2CG5	Fungi	289
137	437	66	0	434	0	0		K0KY39_WICCF		62		Eukaryota	257
138	432	66	0	406	0	0				76		Fungi	222
139	431	87	0	363	0	0		J9VTQ2_CRYNH		89		Fungi	301
140	429	82	0	399	0	0				73		Fungi	259
141	428	66	0	423	4	1	PT repeat: 4		6IU4	60		Eukaryota	449
142	426	84	0	398	0	0		R9ARJ4_WALI9		83		Fungi	382
143	423	63	0	421	0	0		N4VW89_COLOR		58		Universal	716
144	418	86	0	375	0	0		L8WX25_THACA		88		Fungi	205
145	417	83	0	399	0	0		A0A074SGL0_9HOMO		85		Fungi and Viridiplantae	316
146	416	82	0	405	0	0				70		Fungi	290
147	414	85	0	388	0	0				82		Fungi and Viridiplantae	634
148	411	62	0	407	0	0		A0A094BMP2_9PEZI		55		Fungi	534
149	409	63	0	409	0	0		A0A0A2KY00_PENIT	5AAZ	63		Eukaryota	603
150	408	86	0	342	0	0				70		Fungi	236
151	408	60	1	405	1	1	Chromatin assembly factor 1 complex p150 subunit, N-terminal: 1			58		Universal	433
152	407	65	0	405	0	0				88		Fungi	370
153	406	86	0	375	0	0		R9PAR4_PSEHS		76		Fungi	157
154	405	64	0	400	0	0		G3J9J4_CORMM		58		Fungi	472
155	404	60	0	398	0	0				74		Fungi	531
156	404	86	0	367	0	0			6IY4	94	1C8Z	Fungi	182
157	403	83	0	388	319	1	WD domain, G-beta repeat: 319		4J87	89		Fungi	372
158	399	83	0	385	0	0		K1WTR5_TRIAC		75		Fungi	309
159	398	64	0	393	5	1	Meiosis-specific protein Mei4: 5	A0A0L1HHG0_9PLEO		72		Fungi and Viridiplantae	514
160	398	70	0	386	0	0				79		Fungi and Viridiplantae	524
161	395	84	0	356	0	0				94		Fungi and Viridiplantae	208
162	394	73	0	148	0	0				73		Fungi and SAR	222
163	394	84	0	374	0	0		L5LYU0_MYODS		87		Fungi	232
164	393	86	0	349	0	0		E6N947_9ARCH		71		Fungi	316
165	392	88	0	378	0	0		A0A0B1P0W1_UNCNE		81		Fungi	197
166	391	71	0	383	0	0				74		Fungi	406
167	390	88	0	364	0	0		E6X145_NITSE		52		Fungi and Viridiplantae	470
168	389	87	0	362	1	1	Heme oxygenase: 1	A0A074S9A8_9HOMO		78		Fungi	230
169	387	62	0	383	0	0				44		Fungi	1,052
170	386	87	0	362	0	0		S3CYU6_OPHP1	4FO1	94	5UVD	Fungi	194
171	386	85	0	364	2	1	SUR7/PalI family: 2			88	6OV3	Fungi	235
172	385	85	0	370	192	2	V-type proton ATPase subunit S1, luminal domain: 191 PF08319: 1		6VQ6	80		Fungi	281
173	385	63	0	364	0	0				55		Fungi and SAR	603
174	384	64	0	381	0	0				50		Fungi	325
175	384	86	0	378	0	0				60		Fungi	782
176	382	82	0	368	168	3	Ankyrin repeats (3 copies): 139 Ankyrin repeat: 29 Ankyrin repeats (many copies): 2		1OY3	86		Fungi	272
177	381	82	0	234	6	2	EthD domain: 4 Antibiotic biosynthesis monooxygenase: 2		2FTR	95		Universal	235
178	380	62	0	370	2	1	Chromatin assembly factor 1 complex p150 subunit, N-terminal: 2			49		Fungi	481
179	380	86	0	375	0	0				71		Universal	383
180	378	66	0	377	1	1	AT hook motif: 1			77		Fungi	167
181	374	89	0	358	0	0		A0A0L1HZV7_9PLEO		80		Fungi	247
182	373	85	0	364	0	0			6UP7	78		Fungi	532
183	372	88	0	364	0	0				60		Fungi	393
184	371	64	0	369	0	0				81		Fungi	252
185	371	88	0	335	0	0				85		Fungi	143
186	371	87	0	354	0	0		A0A0B4GRS7_9HYPO	3DKQ	76		Fungi	482
187	370	71	0	368	0	0				53		Fungi	347
188	370	88	0	336	2	2	Nitrate and nitrite sensing: 1 Putative transmembrane protein: 1			79		Fungi	203
189	370	87	0	364	0	0				63		Fungi	395
190	369	85	0	354	0	0		A0A074S9U8_9HOMO		72		Fungi	371
191	368	62	0	367	0	0		A0A0F8CWX3_CERFI		77		Fungi	138
192	367	87	0	360	0	0			5MRF	88		Fungi	372
193	366	60	0	364	0	0		A0A0K8L317_9EURO		72		Eukaryota	193
194	365	87	0	337	0	0				93		Fungi	177
195	364	85	0	344	0	0				64		Fungi	694
196	364	84	0	344	0	0				59		Fungi	246
197	364	85	0	340	29	3	Leucine Rich repeat: 20 Leucine Rich Repeat: 8 Leucine Rich Repeat: 1		1G9U	82		Fungi	714
198	363	75	0	361	0	0		A0A0C9MWL6_9FUNG		70		Fungi and Viridiplantae	664
199	363	87	0	350	0	0				62		Fungi	451
200	359	84	1	345	0	0				80		Fungi and Viridiplantae	348
201	359	89	0	342	1	1	Protein of unknown function (DUF724): 1		6F1T	58		Fungi and Viridiplantae	925
202	358	86	0	352	0	0				84		Fungi	296
203	357	87	0	355	0	0		K1W1R5_TRIAC		51		Fungi and Viridiplantae	948
204	356	54	0	304	0	0		N0DR50_9HYPO		53		Fungi	448
205	355	87	0	345	0	0				59		Fungi	863
206	355	88	0	338	0	0		A0A0K6FW51_9HOMO		79		Fungi	674
207	355	85	0	349	0	0			1G2R	83		Fungi	437
208	355	84	0	318	0	0				53		Fungi and Viridiplantae	334
209	354	85	0	353	0	0		L8WNI9_THACA		79		Fungi and Viridiplantae	527
210	353	72	0	352	0	0				75		Eukaryota	260
211	352	86	0	332	0	0		L8WGA7_THACA		57		Fungi	213
212	350	86	0	327	4	3	Protein of unknown function (DUF1180): 2 Ykl077w/Psg1 (Pma1 Stabilization in Golgi): 1 Glycophorin A: 1		2KNC	67		Fungi and Viridiplantae	428
213	350	84	0	344	0	0				88		Fungi	211
214	349	88	0	346	0	0				60		Fungi and Viridiplantae	392
215	349	87	0	342	0	0				82		Fungi	215
216	347	64	0	335	0	0				71		Fungi and Viridiplantae	136
217	346	84	0	335	0	0			6K15	57		Fungi and Viridiplantae	723
218	344	86	0	336	0	0				57		Fungi	225
219	343	87	0	329	0	0				83		Fungi	520
220	341	85	0	338	1	1	HSCB C-terminal oligomerisation domain: 1			68		Fungi and Viridiplantae	962
221	340	87	0	331	0	0		J5R5X1_TRIAS		87		Fungi	152
222	338	87	0	336	0	0				49		Fungi	434
223	338	89	0	335	0	0				72		Fungi	313
224	337	63	0	334	0	0		A0A0K8LB55_9EURO	6TDZ	72		Fungi and Viridiplantae	1,019
225	337	73	0	336	0	0		A0A0F4Z6D9_TALEM		75		Fungi	194
226	335	88	0	330	0	0		V5FD21_BYSSN		94		Fungi	130
227	334	86	0	315	0	0				58		Fungi	480
228	331	66	0	328	0	0				56		Fungi	263
229	330	88	0	328	3	1	Transient receptor potential (TRP) ion channel: 3			76		Eukaryota	1,157
230	330	86	0	330	0	0				72		Fungi and Viridiplantae	516
231	328	87	0	306	0	0		A0A0K6FXY4_9HOMO		71		Fungi and Viridiplantae	536
232	328	63	0	325	0	0				49		Fungi	501
233	328	86	0	324	0	0				45		Fungi	992
234	327	85	0	324	0	0				54		Fungi and Viridiplantae	300
235	323	88	0	321	1	1	F-box domain: 1	L8WMQ0_THACA	3ZYO	88		Fungi	434
236	323	72	0	322	1	1	Collagen triple helix repeat (20 copies): 1	A0A0L1HFE7_9PLEO		66		Fungi and Viridiplantae	463
237	319	63	0	318	0	0		S3C2N9_OPHP1		52		Fungi	453
238	318	84	0	312	0	0				60		Fungi	220
239	318	87	0	308	0	0				87		Fungi	341
240	314	85	0	305	72	1	DASH complex subunit Spc34: 72			64		Fungi	508
241	313	65	0	310	0	0				48		Fungi	679
242	311	67	0	308	0	0		V5FZN3_BYSSN		51		Fungi and SAR	363
243	309	61	0	308	0	0				53		Fungi and Viridiplantae	974
244	308	85	0	283	0	0				79		Fungi	153
245	307	84	1	307	0	0				66		Fungi and Prokaryotes	473
246	306	71	0	304	0	0		A0A094BMP2_9PEZI		57		Fungi and Viridiplantae	446
247	305	65	0	304	0	0			4N6J	58		Fungi	425
248	305	88	0	299	0	0				64		Fungi	155
249	303	86	0	290	19	1	Glutaredoxin: 19	A0A0L6WBI9_9AGAR	2MXN	80		Fungi and Viridiplantae	267
250	302	58	0	301	0	0		A0A0L1HDJ6_9PLEO		65		Fungi	411
251	300	88	0	292	0	0		T0JIZ7_9FIRM		63		Fungi and Viridiplantae	291
252	298	84	0	296	0	0		A0A0K6G7K8_9HOMO		80		Fungi	628
253	296	65	0	294	1	1	Porin subfamily: 1			71		Fungi	372
254	295	64	0	293	0	0				59		Fungi	160
255	294	65	0	293	0	0		A0A0F0IGJ6_ASPPA		56		Fungi	324
256	293	65	0	293	0	0		A0A0B1P0W1_UNCNE		69		Fungi	293
257	291	65	0	290	0	0				67		Fungi and SAR	235
258	291	87	0	273	159	2	Ykl077w/Psg1 (Pma1 Stabilization in Golgi): 158 Amino acid permease: 1	A0A074RYD1_9HOMO		59		Fungi	516
259	291	65	0	290	0	0		R8BUG0_TOGMI		54		Universal	393
260	288	48	0	245	0	0				89		Eukaryota	262
261	288	86	0	283	0	0		L8WYB1_THACA		63		Fungi	227
262	285	65	0	285	0	0				44		Fungi	877
263	284	71	0	278	3	1	AT hook motif: 3	Q91795_XENLA	6F1T	63		Fungi and SAR	668
264	283	71	0	283	0	0		A0A0G2EQR6_9EURO		62		Fungi	245
265	282	87	0	269	1	1	Alba: 1		4Z9E	80		Fungi	209
266	282	88	0	277	0	0			6JLB	48		Fungi and Viridiplantae	1,109
267	281	88	0	275	0	0				72		Fungi	361
268	281	89	0	281	0	0				80		Fungi	203
269	280	89	0	272	2	2	UbiA prenyltransferase family: 1 Tetraspanin family: 1			70		Fungi	303
270	280	66	0	280	0	0				60		Fungi and Viridiplantae	468
271	279	88	0	269	0	0				72		Fungi and Viridiplantae	360
272	279	66	0	279	0	0				64		Fungi	652
273	277	79	0	276	0	0				73		Fungi	213
274	275	85	0	266	0	0		M3IUQ5_CANMX	5N9J	61		Fungi	370
275	274	87	0	266	0	0		A0A0K6GID5_9HOMO	4WBQ	80		Fungi	467
276	271	69	0	269	0	0				51		Fungi	410
277	269	72	0	269	0	0				71		Fungi	331
278	268	83	0	236	0	0				78		Fungi	402
279	265	70	0	263	0	0				67		Fungi	158
280	264	88	0	262	2	1	Nematode cuticle collagen N-terminal domain: 2			58		Fungi and Viridiplantae	568
281	262	92	0	260	0	0				54		Fungi and Viridiplantae	933
282	262	89	0	259	0	0				77		Fungi	155
283	258	72	0	258	0	0			6QLE	62		Fungi	610
284	256	75	0	256	0	0				62		Fungi	824
285	255	69	0	254	0	0				88		Fungi	140
286	254	50	0	245	15	1	F-box domain: 15		4I6J	76		Universal	493
287	252	86	0	246	2	2	Amastin surface glycoprotein: 1 PMP-22/EMP/MP20/Claudin tight junction: 1		6C14	87	7OCD	Fungi	216
288	250	82	0	250	8	3	EF-hand domain pair: 8 EF hand associated: 6 EF hand associated: 6	M9LV43_PSEA3	4C0J	79		Fungi and Viridiplantae	153
289	246	85	0	243	0	0				62		Fungi	306
290	243	88	0	239	0	0			5X6O	65		Fungi	813
291	241	72	0	236	0	0				68		Fungi and Viridiplantae	168
292	238	76	0	238	0	0				57		Fungi and Viridiplantae	274
293	237	41	0	140	0	0				91		Fungi	253
294	236	78	0	236	1	1	Dephospho-CoA kinase: 1			52		Fungi	549
295	236	76	0	235	0	0				68		Fungi	344
296	235	88	0	233	0	0				48		Fungi	829
297	234	79	0	233	0	0		A0A093UVH3_TALMA	4Y0G	52		Fungi and Viridiplantae	628
298	232	45	0	147	0	0		V2X8U2_MONRO		80		Fungi and Viridiplantae	965
299	231	85	0	210	0	0		X8JEG6_9HOMO	1UG0	71		Fungi	315
300	226	84	0	205	17	1	Ricin-type beta-trefoil lectin domain: 17		4FBR	83		Fungi	270
301	222	92	0	209	0	0			4J8Z	71		Fungi	297
302	220	88	0	218	0	0				68		Fungi	557
303	217	78	0	206	0	0		A0A0K6GIN9_9HOMO		85		Fungi	205
304	214	80	0	213	0	0				55		Fungi	1,193
305	212	88	0	207	0	0		L8WN56_THACA		64		Fungi and Viridiplantae	581
306	211	53	0	204	0	0				71		Fungi and Viridiplantae	179
307	211	46	0	167	0	0			4DIM	84		Universal	552
308	210	68	0	197	0	0			5XJO	88		Fungi and Viridiplantae	481
309	205	60	0	201	9	1	Nuclear pore complex subunit Nro1: 9		3MSV	85		Fungi and Prokaryotes	247
310	204	84	0	204	0	0				74		Fungi and SAR	370
311	203	71	0	193	0	0			6IYX	93		Fungi	285
312	201	63	0	152	0	0				70		Fungi	396
313	199	87	0	199	0	0				44		Fungi and Viridiplantae	1,131
314	197	26	0	131	0	0				86		Fungi and opisthokonts	153
315	182	32	0	125	0	0		V5FD21_BYSSN		87		Fungi	124
316	174	83	0	160	0	0				82		Fungi	210
317	169	66	0	166	0	0				76		Fungi and Viridiplantae	335
318	164	65	0	160	0	0				67		Fungi and Viridiplantae	175
319	155	67	0	152	0	0		A0A0Q3Y684_ALLMI	4XPM	80		Fungi and Viridiplantae	219
320	151	32	0	129	0	0			5LDQ	90		Universal	210
321	151	68	0	143	0	0		A0A0D2F0Y5_9EURO		52		Fungi	230
322	150	66	0	146	0	0				75		Fungi	340
323	150	69	0	144	0	0		A0A0A0HS69_PARBD		78		Fungi and Prokaryotes	173
324	149	65	0	143	0	0				85		Fungi	197
325	147	55	0	143	0	0		K2RK39_MACPH		71		Fungi and Viridiplantae	406
326	144	69	0	142	0	0		M2Y9N8_GALSU		82		Fungi and Viridiplantae	729
327	144	72	0	142	0	0		A0A0F0I897_ASPPA		67		Fungi and Viridiplantae	182
328	143	69	0	138	0	0		W9C530_9HELO		69		Fungi	105
329	142	77	0	127	0	0		A0A0P1BS65_9BASI		73		Fungi	137
330	141	40	0	128	1	1	KH domain: 1			81		Fungi	636
331	140	46	0	139	0	0				77		Eukaryota	245
332	136	70	0	133	0	0				67		Fungi and Prokaryotes	232
333	130	28	0	112	0	0				77		Fungi	152
334	119	27	0	104	0	0		D3AXE7_POLPA	6QB2	82	1STF	Fungi	123
335	118	36	0	116	0	0				79		Fungi	326
336	115	67	0	103	0	0				64		Fungi	211
337	110	31	0	107	14	2	HEAT repeat: 11 HEAT repeat associated with sister chromatid cohesion: 3	A0A081BYL9_9BACT	4RV1	83		Fungi	814
338	106	76	0	106	0	0		C0P0H6_AJECG		56		Fungi	398
339	102	25	0	102	0	0		L0LKP3_RHITR		88		Fungi and opisthokonts	169

Methods

Over 18 millions proteins encoded in 1282 fungal genomes from Mycocosm were clustered into families using cascaded MMseqs2 with default parameters (Steinegger et al, 2017). Our subset of 142 clusters have the following 3 properties. Each is:

conserved across large phylogenetic distances, i.e. present in either (i) >50% of all fungal genomes, or (ii) >90% of genomes in the clade named in the 'Conserved in' column.
of unknown function, i.e. have few or no (i) known Pfam domains except domains without specific function, like those starting with DUF or UPF, nor (ii) functionally annotated Blastp hits against Swissprot.
encoded by expressed genes, i.e. >20% genes are supported by transcriptomics data.

An individual family member may have manual curations retrieved from MycoCosm or functional domains not shared with the rest of its family. Families as a whole may also have similarity to distant protein families in Uniprot or Protein Data Bank (PDB), as found by pairwise HMM-based HHblits searches (Steinnegger et al, 2019) against the non-redundant Uniprot20_2016 (defined by <20% sequence identity) and PDB70 (defined by <70% sequence identity) sets of protein sequences. Such distantly related proteins are presented in the list as "hints" (‘Uniprot HMM Hint’ and ‘PDB HMM Hint’ columns).

Methods

References