Status

v2.0 (February 15, 2008): The finished version of Micromonas strain RCC299 v2.0 contains 17 chromosomes represented telomere to telomere with 5 gaps and a total genome size of 21 Mbp. The chromosomes have been numbered 1-17, largest to smallest. A total of 10,056 gene models were predicted and functionally annotated using the JGI annotation pipeline.

v1.0: The assembly release version 1.0 of whole genome shotgun reads was constructed with the JGI assembler, Jazz, using paired end sequencing reads at a coverage of 8.9x. After trimming for vector and quality, 274,180 reads assembled into 251 scaffolds totaling 22.3 Mb. Roughly half of the genome is contained in 7 scaffolds all at least 1.4 Mb in length. The v. 1.0 assembly includes a total of 8,007 gene models predicted and functionally annotated using the JGI annotation pipeline.

Collaborators

• US Department of Energy Joint Genome Institute (JGI)
• Alexandra Z. Worden, Monterey Bay Aquarium Research Institute

Links

• Micromonas sp.RCC299

Movie

• Paraphysomonas grazing on M. pusilla NOUM17 (mpg): Micromonas are red (a function of chlorophyll fluorescence from their chloroplasts) and can be seen swimming in and out of the field. The greenish particles are the stained food vacuoles of Paraphysomonas, a eukaryotic heterotrophic microbe or “grazer”. The red/orange in the “gut” of Paraphysomonas are ingested Micromonas cells The acidic food vacuole of Paraphysomonas is stained with Lysotracker Green. Contributed by the Worden Lab (H. Moehlig).

Funding

This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396.

Funding for preparation of cell lines, genomic and transcriptomic material as well as manual annotation was provided by a Gordon & Betty Moore Young Investigator Award in Marine Microbiology to A.Z. Worden.